General Cell Based Assays
Bioassay Systems EnzyChrom™ L-Alanine Assay Kit. For quantitative determination of L-Alanine and evaluation of drug effects on alanine metabolism.
For quantitative determination of L-Alanine and evaluation of drug effects on alanine metabolism. Key Features: Sensitive and accurate. Linear detection range in 96-well plate: 1 to 200 µM alanine for colorimetric assays and 0.4 to 20 µM for fluorimetric assays. Method: OD570nm, or FL530/585nm. Samples: Plasma, serum, urine, tissue and culture media. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 0.4 µM.
Eurofins Discoverx PathHunter eXpress IL17RA/IL17RC Dimerization Assay, 2-Plate Kit. (93-0999E3CP0M)
The PathHunter IL17RA/IL17RC (U2OS) Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-17a) induced dimerization of IL17RA/IL17RC (U2OS) receptors. This product uses EFC technology. The cell line included overexpresses IL17RC target protein fused to PK alongside IL17RA target protein fused to EA. Upon ligand-induced dimerization of IL17RA/IL17RC, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Mouse Fractalkine (CX3CR1) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Mouse Fractalkine (CX3CR1) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure mCX3CR1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Cell Biolabs Inc Total Bile Acid Assay Kit (Fluorometric), 100 assays
Determining circulatory levels of bile acids can be used to identify or diagnose certain liver diseases. In addition, elevated serum bile levels have been observed in intrahepatic cholestasis of pregnancy cases. Cell Biolabs’ Total Bile Acid Assay Kit is a simple fluorometric assay that measures the amount of total bile acid present in plasma, serum, tissue homogenates, or cell lysates in a 96-well microtiter plate format. The assay is based on an enzyme driven reaction: when bile acids are incubated in the presence of 3α-hydroxysteroid dehydrogenase (3α-HSD) and NAD+, NAD+ is converted to its reduced form NADH. Diaphorase then uses NADH to reduce resazurin to resorufin which is then detected fluorometrically. Sample bile acid concentrations are determined by comparison with a known bile acid standard. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, bile acid standards and unknown samples.
Eurofins Discoverx PathHunter Human IGF1R Functional Kinase Cell-Based Assay Kit (HEK 293)
The PathHunter Human IGF1R Stable Cell Line Functional Kinase Assay (HEK 293) measures IGF1R receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx InCELL Hunter eXpress HEK 293 BRD3(1,2) Bromodomain Assay, 10-plate Kit. (96-0038E1CP7L)
The InCELL Hunter eXpress HEK 293 BRD3(1,2) Bromodomain Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with regulators of epigenetics pathways. This kit assesses compound-engagement-based stabilization of BRD3(1,2) via detection of BRD3(1,2) levels. This product uses EFC technology. The cell line included overexpresses BRD3(1,2) target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter™ Human SDF-1 alpha (CXCR4) Gi Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human SDF-1 alpha (CXCR4) Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure CXCR4 (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Meso Scale Discovery U-PLEX Mouse IL-9 ASY
The Meso Scale Discovery U-PLEX Mouse IL-9 Assay Kit is an electrochemiluminescent immunoassay for the quantitative measurement of mouse interleukin 9 (IL-9) in serum plasma and cell culture supernatants The assay uses MSD s proprietary SULFO-TAG labels and multi-array technology to provide sensitive and reproducible quantitation of mouse IL-9 with a lower limit of detection of 0 3 pg/mL and an upper limit of 10 000 pg/mL The kit includes a 96-well plate coated with capture antibody detection antibody conjugated to MSD SULFO-TAG calibrators and sample diluent The assay takes approximately 3 5 hours to complete after a 2 hour incubation
Revvity Health Sciences Inc AlphaScreen FITC Detection Kit, 50,000 Assay Points
AlphaScreen FITC Detection Kit, 50,000 Assay Points
ABclonal Technology Phospho-CRMP2/DPYSL2-T514 Rabbit pAb
This gene encodes a member of the collapsin response mediator protein family. Collapsin response mediator proteins form homo- and hetero-tetramers and facilitate neuron guidance, growth and polarity. The encoded protein promotes microtubule assembly and is required for Sema3A-mediated growth cone collapse, and also plays a role in synaptic signaling through interactions with calcium channels. This gene has been implicated in multiple neurological disorders, and hyperphosphorylation of the encoded protein may play a key role in the development of Alzheimers disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Cayman Chemical Triglyceride Colorimetric Ass
Cayman’s Triglyceride Colorimetric Assay Kit provides a simple, reproducible, and sensitive tool for assaying mono-, di-, and triglycerides in plasma, serum, cell culture extracts, and tissue homogenates. The Triglyceride Colorimetric Assay Kit uses the enzymatic hydrolysis of triglycerides by lipase to produce glycerol and free fatty acids. The glycerol released is subsequently measured by a coupled enzymatic reaction system. The glycerol formed in reaction 1 is phosphorylated to glycerol-3-phosphate in a reaction catalyzed by glycerol kinase. The glycerol-3-phosphate is oxidized by glycerol phosphate oxidase producing dihydroxyacetone phosphate and hydrogen peroxide (H2O2). Peroxidase catalyzes the redox-coupled reaction of H2O2 with 4-aminoantipyrine (4-AAP) and N-ethyl-N-(3-sulfopropyl)-m-anisidine (ESPA), producing a brilliant purple color. The absorbance is measured at 540 nm.
STEMCELL Technologies Ascorbic Acid, Size: 100 g
Support the proliferation of mesenchymal stem cells (Choi et al. J Biosci Bioeng, 2008) with Ascorbic Acid. Ascorbic Acid is a reducing agent that shows antioxidant activity against reactive oxygen species. Ascorbic Acid is used to differentiate mouse embryonic stem (ES) cells into osteoblasts (zur Nieden et al. Differentiation, 2003) and cardiomyocytes (Takahashi et al. Circulation, 2003). This small molecule also promotes differentiation of osteoblasts from human and mouse mesenchymal cells (Pittenger et al. Science, 1999; Tropel et al. Exp Cell Res, 2004) and increases the efficiency of reprogramming mouse and human fibroblasts to induced pluripotent stem (iPS) cells (Esteban et al. Cell Stem Cell, 2010). L-Ascorbic Acid is supplied as a crystalline solid with ≥95% purity; Molecular weight 176.1 g/mol; CAS Number 50-81-7. Visit STEMCELL.com for more information and related products.
Eurofins Discoverx PathHunter Human EphA4 Functional Kinase Cell-Based Assay Kit (U2OS)
The PathHunter Human EphA4 Stable Cell Line Functional Kinase Assay (U2OS) measures EphA4 receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.